Taq dna polymerase is the industry standard for routine pcr. Platinum taq dna polymerase high fidelity contains recombinant taq dna polymerase, pyrococcus species gbd polymerase, and platinum taq antibody. The five quality features of q5 high fidelity dna polymerase 1. Taq dna polymerase is an enzyme widely used in pcr 2. Smarter pcr with platinum ii taq dna polymerase scientific. Janica b a central forensic laboratory of the police, warsaw, poland b department of forensic medicine, medical university of bialystok, poland abstract. Efficiency comparison of seven different taq polymerases. Accuprime taq high fidelity contains a proprietary mixture of taq polymerase, taq, invitrogen, 47, platinum 19, amplitaq 10. A universal primer annealing feature reduces optimization steps and allows for cocycling of all assays. Efficiency comparison of seven different taq polymerases used in hemogenetics j. Taq dna polymerase invitrogen platinum 120 reaction. Protocol the procedure on the following page is suggested as a guideline and starting point when using platinum taq dna polymerase in any pcr amplification. For most routine andor at rich amplicons or complex invitrogen platinum taq 302, 94c ab.
A unique combination of innovative buffer, highperformance taq dna polymerase, and superior hotstart technology enables. Low glycerol platinum taq, cg dna polymerase is a recombinant taq polymerase complexed with a. Usually, this is done for the pcramplified dna after round 7 or later selection rounds, depending on the enrichment of the binders. Pcr platinum pfx dna polymerase polymerase description proofreading 3 a 5 exonuclease activity fast chain extension capability one unit of pfx pol incorporates 10 nmol of dntps in 30 min at 74. Platinum hotstart technology inhibits dna polymerase activity at ambient temperatures, allowing room temperature. Featuring greater than 100x taq fidelity, platinum superfi dna polymerase. Optimization strategies click here for guidelines to optimize your pcr experiment. Invitrogen platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase.
Faststart taq dna polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. Invitrogen platinum taq dna polymerase 600 reactions. Dna polymerase delivered both greater intensity amplicons and amplification success in cases where standard. Invitrogen platinums taq dna polymerase high fidelity. Like regular taq, platinum taq dna polymerase high fidelity has a nontemplatedependent terminal transferase. Please provide your institution address, samples will not be sent to homepersonal addresses. Pcr protocol for longamp taq dna polymerase m0323 overview. Platinum superfi dna polymerase 100x taq fidelity for 100% confidence invitrogen platinum superfi dna polymerase is a proofreading dna polymerase that combines exceptional fidelity with trusted platinum hotstart technology, offering the highest success in pcr. Equipment 96well plate magnetic stand invitrogen qubit fluorometer 2. Invitrogen platinum ii taq hotstart dna polymerase 2500 rxns invitrogen platinum ii taq hotstart dna polymerase hot start pcr. Platinum taq dna polymerase high fidelity 20l, at 5ul is supplied with a vial of 10x high fidelity buffer 600mm trisso 4 ph 8.
Pcr protocol for longamp taq dna polymerase m0323 neb. Superscriptz iii onestep rtpcr system with platinum. Pcr protocol see page 2 for instructions to prepare and run your pcr experiment. Primers formed dimers design primers without complementary sequences at the 3 ends. It is a single polypeptide chain with a molecular weight of approximately 95 kda. No modification to pcr reactions or protocols are necessary. Platinum taq high fidelity should create a mixture of pcr products with extra a thanks to taq and some blunt ended thanx to the. Pcr protocol for taq dna polymerase with standard taq buffer m0273. Benefits of invitrogen platinum ii taq hotstart dna polymerase. A protocol for the use of gotaq dna polymerase with 5x green gotaq reaction buffer and 5x colorless gotaq reaction buffer. Taq mix in the reaction nonspecific annealing of primers vary the annealing temperature, optimize the magnesium concentration for each template and primer combination. Use platinum taq dna polymerase for the amplification of dna from complex genomic, viral, and plasmid templates, as well as in rtpcr. Mar 05, 2019 simpler, cleaner, smarterthese are some pcr benefits you can achieve with invitrogen platinum ii taq hotstart dna polymerase.
Invitrogen platinum ii taq hotstart dna polymerase is designed for universal primer annealing and fast, easy pcr with its unique combination of innovative buffer, highperformance engineered taq dna polymerase, and superior hotstart technology. The enzyme is a taq dna polymerase with buffers designed for enhanced amplification. Platinum ii taq dna polymerase provides higher pcr specificity due. Platinum taq dna polymerase high fidelity offers the best of both yield and fidelity and therefore, is a good choice when quantity and quality are important. Taq dna polymerase was originally isolated from the thermophilic eubacterium thermus aquaticus bm, a strain lacking taq i restriction endonuclease. Platinum taq dna polymerase high fidelity is supplied at the same 5 unit per l concentration as platinum taq dna polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render taq dna polyme. Smarter pcr with platinum ii taq dna polymerase youtube. Phage was probably talking about platinum taq high fidelity which indeed is a mixture of taq and a proofreader not pfu, pfu is sold by fermentas, stratagene and promega, but not by invitrogen, they have pfx. Platinum taq dna polymerase, high fidelity, is ideal for amplification of dna fragments when high yields and robust amplification are.
Simpler, cleaner, smarterthese are some pcr benefits you can achieve with invitrogen platinum ii taq hotstart dna polymerase. Neb provides high quality recombinant taq at an exceptional value. C avoids the necessity of thawing the mix before assembling the reaction. Invitrogen platinum taq dna polymerase 300 reactions. Invitrogen platinum ii taq hotstart dna polymerase 25. Currently, str typing is the most efficient and the fastest way for identification of. Platinum taq dna polymerase is a convenient and reliable. Invitrogen platinum taq dna polymerase high fidelity.
It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. After pcr using taq polymerase, the fragments are cloned into plasmids by ta cloning taq amplified for sequencing. For multiple reactions, prepare a master mix of components common to all. Platinum taq dna polymerase high fidelity protocol 20142platinum taq dna polymerase high fidelity protocol the example pcr procedure below shows appropriate volumes for a single 50l reaction. G501801 invitrogen trackit 1 kb plus dna ladder cat. A wide range of pcr products can be generated, up to 30 kb from lambda dna or. I posted a problem earlier about my pcr product not migrating from the agarose gel well. Invitrogen platinum ii taq hotstart dna polymerase 2500. Tm is defined as at this temperature half of the dna duplex will dissociate to become. Invitrogen platinum pfx enzyme not so fantastic protocol online. Platinum taq dna polymerase is composed of recombinant taq dna polymerase, pyrococcus gb. C and is recommended for use in pcr and primer extension reactions that require high fidelity. The oral microbiome is comprised of over 600 prevalent taxa at the species level, with distinct subsets predominating at different habitats.
Taq dna polymerase is a thermostable dna polymerase that possesses a 5. Invitrogen platinum taq ii hotstart dna polymerase is designed for universal primer annealing and fast, easy pcr with its unique combination of innovative buffer, highperformance engineered taq, and leading hotstart technology. Invitrogen platinum ii hotstart pcr master mix 2x 50 rxns invitrogen platinum ii hotstart pcr master mix 2x pcrreagentia. Hi adnan, one step rtpcr kit includes chemical conditions for platinum taq amplification, which uses cl2 as cation donor. Invitrogen platinum pfx enzyme not so fantastic problems with pcr product gel migration aug102004 hi. Taq dna polymerase taq hot start dna polymerase onetaq dna polymerase is supplied with two 5x buffers standard and gc, as well as a high gc enhancer solution. Invitrogen platinum taq dna polymerase fisher scientific. The human oral cavity contains a number of different habitats, including the teeth, gingival sulcus, tongue, cheeks, hard and soft palates, and tonsils, which are colonized by bacteria. Invitrogen platinum taq dna polymerase is a convenient and reliable. Takara taq dna polymerase is a recombinant version of the thermostable, fulllength taq polymerase derived from the thermus aquaticus yt1 strain, which is suitable for routine pcr applications. The oral microbiome has been extensively characterized by. Platinum taq dna polymerase and increasing the extension time as specified 1 min per kb.
Mar 08, 2006 platinum taq dna polymerase high fidelity offers the best of both yield and fidelity and therefore, is a good choice when quantity and quality are important. Dynabeads magnetic separation technology, gibco cell culture media and reagents, superscript reverse transcriptase, platinum taq polymerase, topo cloning and expression products, novex protein electrophoresis products, and numerous fluorescent reagents such as. Platinum taq dna polymerase from thermo fisher scientific. Moreover, platinum ii taq hotstart dna polymerase is a fast dna polymerase.
Platinum taq dna polymerase from thermo fisher scientific description platinum taq dna polymerase is a convenient and reliable hot start thermostable dna polymerase for pcr that provides enhanced specificity over that of taq dna polymerase. Superscript iii onestep rtpcr system with platinum taq. This enzyme allows amplification of simple and complex dna templates over a large range of target sizes and provides 6x higher fidelity over taq. Platinum taq, 3 a and proofreading molecular biology. Im thinking between invitrogens platinum taq hotstart, kapa biosystems hifi hotstart and neb. Platinum ii taq dna polymerase is a hotstart enzyme used for robust.
In the early stages of pcr development, it became clear that reaction. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step convenience. What is the best highfidelity polymerase to use for subsequent sequencing and protein expression. Faster pcr with platinum ii taq dna polymerase youtube. Taq is available with different formats to accommodate a variety of pcr applications. Platinum superfi dna polymerase is a proofreading dna polymerase that combines fidelity with platinum hotstart technology, and is ideally suited for cloning, mutagenesis, and other applications. Pcr protocol for taq dna polymerase with standard taq buffer m0273 protocols. Taq dna polymerase invitrogen platinum 120 reaction fisher scientific 10966018. Description the superscriptz iii onestep rtpcr system with platinum.
Platinum ii taq hotstart dna polymerase thermo fisher scientific. Taq dna polymerase is designed for the sensitive, reproducible, endpoint detection and analysis of rna molecules by rtpcr. Platinum taq dna polymerase is composed of recombinant taq dna polymerase, pyrococcus gbd polymerase and platinum taq antibody. Jun 24, 2015 discover how you can reduce nonspecific amplification and increase yield by using a hotstart pcr enzyme such as invitrogen platinum taq dna polymerase. Takara taq polymerase has the same characteristics and capabilities as native taq dna polymerase, including 53 exonuclease activity.
For multiple reactions, prepare a master mix of components common to all reactions to minimize pipetting error, then dispense appropriate volumes into each 0. Highest fidelity dna amplification available at 280x higher than taq, q5 offers unparalleled fidelity for your most important samples, but with a protocol and pricepoint that makes it accessible. This enzyme formulation can also be used in larger volume cocktail mixes without difficulty. Efficient, convenient, fastthese are some pcr benefits you can achieve with invitrogen platinum ii taq hotstart dna polymerase. Pcr the polymerase chain reaction pcr is a powerful and sensitive technique for dna amplification 1. Platinum taq dna polymerase2the example pcr procedure below shows appropriate volumes for a single 50l reaction. The hot start property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render taq dna polymerase inactive until the initial pcr denaturation step. Invitrogen platinum ii hotstart pcr master mix 2x 50. Platinum ii taq hotstart dna polymerase thermo fisher. Platinum taq dna polymerase thermo fisher scientific. Hot start taq polymerase invitrogen taq polymerase. Longamp taq dna polymerase offers two fold higher fidelity than taq dna polymerase alone. Taq dna polymerase, originally isolated from thermus aquaticus, is most commonly used in pcr assays 1.
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